Activation and Regulation of Blood Vδ2 T Cells Are Amplified by TREM-1+ during Active Pulmonary Tuberculosis

J Immunol. 2018 Mar 1;200(5):1627-1638. doi: 10.4049/jimmunol.1700785. Epub 2018 Feb 2.

Abstract

Triggering receptor expressed on myeloid cells 1 (TREM-1) is a receptor mainly expressed on myeloid cells, and it plays an important role in modulating immune response against infectious agents. The function of TREM-1 on nonmyeloid cells such as Vδ2 T cells has not been characterized, and their role in pulmonary tuberculosis (TB) remains unclear. To assess the expression of TREM-1 on blood Vδ2 T cells from pulmonary TB patients and investigate its mechanism of induction, we exploited flow cytometry analysis to study the expression of TREM-1 on Vδ2 T cells from active pulmonary TB patients and control subjects. In this study we demonstrate that TREM-1 (TREM-1+) is highly expressed on Vδ2 T cells of patients with active pulmonary TB. Unlike TREM-1--expressing Vδ2 T cells, TREM-1+-producing Vδ2 T cells display APC-like phenotypes. Surprisingly, TREM-1+ signaling promotes the Ag-presenting capability of Vδ2 T cells to induce the CD4+ T cell response. TREM-1+Vδ2 T cells induced the proliferation and differentiation of naive CD4+ T cells, as well as the elimination of intracellular mycobacteria. We identified TREM-1+ (but not TREM-1-) as an Ag-presentation amplifier on human blood Vδ2 T cells, and data shed new light on the regulation of Vδ2 T cells in the phase of innate and adaptive immune responses against Mycobacterium tuberculosis infection. Targeting TREM-1+Vδ2 T cells may be a promising approach for TB therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptive Immunity / immunology
  • Adult
  • Blood Cells / immunology*
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Differentiation / immunology
  • Cell Proliferation / physiology
  • Cohort Studies
  • Female
  • Humans
  • Immunity, Innate / immunology
  • Lymphocyte Activation / immunology*
  • Male
  • Mycobacterium tuberculosis / immunology
  • Receptors, Antigen, T-Cell, gamma-delta / immunology*
  • Triggering Receptor Expressed on Myeloid Cells-1 / immunology*
  • Tuberculosis, Pulmonary / immunology*

Substances

  • Receptors, Antigen, T-Cell, gamma-delta
  • Triggering Receptor Expressed on Myeloid Cells-1