Targeting Cyclin D-CDK4/6 Sensitizes Immune-Refractory Cancer by Blocking the SCP3-NANOG Axis

Cancer Res. 2018 May 15;78(10):2638-2653. doi: 10.1158/0008-5472.CAN-17-2325. Epub 2018 Feb 6.

Abstract

Immunoediting caused by antitumor immunity drives tumor cells to acquire refractory phenotypes. We demonstrated previously that tumor antigen-specific T cells edit these cells such that they become resistant to CTL killing and enrich NANOGhigh cancer stem cell-like cells. In this study, we show that synaptonemal complex protein 3 (SCP3), a member of the Cor1 family, is overexpressed in immunoedited cells and upregulates NANOG by hyperactivating the cyclin D1-CDK4/6 axis. The SCP3-cyclin D1-CDK4/6 axis was preserved across various types of human cancer and correlated negatively with progression-free survival of cervical cancer patients. Targeting CDK4/6 with the inhibitor palbociclib reversed multiaggressive phenotypes of SCP3high immunoedited tumor cells and led to long-term control of the disease. Collectively, our findings establish a firm molecular link of multiaggressiveness among SCP3, NANOG, cyclin D1, and CDK4/6 and identify CDK4/6 inhibitors as actionable drugs for controlling SCP3high immune-refractory cancer.Significance: These findings reveal cyclin D1-CDK4/6 inhibition as an effective strategy for controlling SCP3high immune-refractroy cancer. Cancer Res; 78(10); 2638-53. ©2018 AACR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle Proteins
  • Cell Line, Tumor
  • Cyclin D1 / antagonists & inhibitors*
  • Cyclin D1 / metabolism
  • Cyclin-Dependent Kinase 4 / antagonists & inhibitors*
  • Cyclin-Dependent Kinase 4 / metabolism
  • Cyclin-Dependent Kinase 6 / antagonists & inhibitors*
  • Cyclin-Dependent Kinase 6 / metabolism
  • DNA-Binding Proteins
  • Female
  • HEK293 Cells
  • Humans
  • Immunotherapy / methods
  • Male
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Nanog Homeobox Protein / genetics
  • Nanog Homeobox Protein / metabolism*
  • Neoplasms / drug therapy*
  • Neoplasms / pathology
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Piperazines / pharmacology*
  • Protein Kinase Inhibitors / pharmacology*
  • Pyridines / pharmacology*
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Zebrafish

Substances

  • CCND1 protein, human
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Nanog Homeobox Protein
  • Nanog protein, mouse
  • Nuclear Proteins
  • Piperazines
  • Protein Kinase Inhibitors
  • Pyridines
  • RNA, Small Interfering
  • Sycp3 protein, mouse
  • Cyclin D1
  • CDK4 protein, human
  • CDK6 protein, human
  • Cyclin-Dependent Kinase 4
  • Cyclin-Dependent Kinase 6
  • palbociclib