No strict requirement for eosinophils for bone marrow plasma cell survival

Eur J Immunol. 2018 May;48(5):815-821. doi: 10.1002/eji.201747229. Epub 2018 Mar 23.

Abstract

Lasting antibody responses are maintained by long-lived plasma cells, which are thought to lodge in the BM in specialized survival niches. Eosinophils have been reported to function as a critical component of the BM survival niche where they are thought to provide pro-survival signals to nearby plasma cells. Recent study shows that many BM plasma cells are recently generated and chiefly short-lived cells, raising the possibility that rare plasma cell-eosinophil interactions are a rate-limiting step needed to establish lasting humoral immunity. To address these issues, we examined the impact of eosinophil depletion on short- and long-lived BM plasma cells in the context of antibody responses induced by both T-cell dependent and T-cell independent antigens. Surprisingly, our results failed to support a role for eosinophils in either plasma cell generation or survival. These studies included examination of plasma cell frequencies in mice lacking eosinophils either after antibody-mediated depletion, or due to mutation of the GATA1 locus.

Keywords: Antibodies; B cells; Eosinophils; Memory; Plasma cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antibody Formation / immunology*
  • Bone Marrow / immunology
  • Bone Marrow Cells / immunology*
  • Eosinophils / immunology*
  • Female
  • GATA1 Transcription Factor / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Plasma Cells / cytology
  • Plasma Cells / immunology*
  • T-Lymphocytes / immunology
  • Tumor Necrosis Factor Ligand Superfamily Member 13 / metabolism

Substances

  • Antibodies
  • GATA1 Transcription Factor
  • Gata1 protein, mouse
  • Tnfsf13 protein, mouse
  • Tumor Necrosis Factor Ligand Superfamily Member 13