Evaluation of the new AspID polymerase chain reaction assay for detection of Aspergillus species: A pilot study

Mycoses. 2018 Jun;61(6):355-359. doi: 10.1111/myc.12757. Epub 2018 Mar 23.

Abstract

The newly developed AspID PCR assay for detection of Aspergillus spp. was evaluated with an interlaboratory quality control programme panel and human bronchoalveolar lavage fluid (BALF) samples. With the quality control programme, 8 out of 9 panel members were correctly identified. With the clinical study, 36 BALF samples that had been obtained from 18 patients with invasive pulmonary aspergillosis (IPA) and 18 without IPA were investigated. Sensitivity, specificity, positive and negative likelihood ratio for the AspID assay were 94.1% (95% CI 73.3-99.9), 76.5% (95% CI 50.1-93.2), 4 (95% CI 1.7-9.5) and 0.1 (95% CI 0.01-0.5) respectively.

Keywords: Asp ID; BALF; PCR; galactomannan; invasive aspergillosis.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antigens, Fungal / genetics
  • Antigens, Fungal / isolation & purification
  • Aspergillosis / diagnosis
  • Aspergillosis / microbiology
  • Aspergillus / genetics
  • Aspergillus / isolation & purification*
  • Bronchoalveolar Lavage Fluid / microbiology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Invasive Pulmonary Aspergillosis / diagnosis*
  • Invasive Pulmonary Aspergillosis / microbiology
  • Male
  • Mannans / analysis
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Molecular Diagnostic Techniques / standards
  • Multiplex Polymerase Chain Reaction / methods*
  • Multiplex Polymerase Chain Reaction / standards
  • Pilot Projects
  • Quality Control
  • Sensitivity and Specificity

Substances

  • Antigens, Fungal
  • Mannans