Urinary neutrophil gelatinase-associated lipocalin (NGAL) and serum cystatin C measurements for early diagnosis of acute kidney injury in children admitted to PICU

World J Pediatr. 2018 Apr;14(2):134-142. doi: 10.1007/s12519-017-0110-x. Epub 2018 Feb 20.

Abstract

Background: Acute kidney injury (AKI) is common in critically ill children with significant mortality and morbidity. Serum creatinine is an insensitive and late biomarker compared to newly proposed AKI biomarkers.

Methods: Prospective study in pediatric intensive care unit (PICU) over three months to compare between serum cystatin-C (s-Cys-C) and urinary neutrophil gelatinase-associated lipocalin (uNGAL) as AKI biomarkers at multiple time points with pediatric risk, injury, failure, loss, end-stage renal disease (pRIFLE) classification in diagnosing AKI.

Results: Forty children were recruited. Of these 40 children, 22 developed AKI according to pRIFLE criteria. There was no significant difference between AKI and non-AKI in age (P = 0.29). Post cardiac surgery, renal insult was the main cause of AKI (27.3%). There was a twofold increased risk of incident AKI in those patients with high baseline uNGAL at PICU admission and almost a fourfold increased risk in patients with high baseline s-Cys-C at PICU admission. uNGAL levels were highly predictive of AKI during the follow-up period [area under the curve (AUC) = 0.76, 95% confidence interval (CI) 0.61-0.92]. The cutoff point with the highest correctly classified proportion was 223 ng/mL (≥ 12 centiles) which correctly predict 80.0% patients with AKI, with a corresponding sensitivity of 72.7% and a specificity of 89.9%. AUC for s-Cys-C was 0.86 (95% CI 0.75-0.97), and the highest correctly classified proportion was 1009 µg/L (≥ 13 centiles); 75% of patients with AKI, with a corresponding sensitivity of 63.6% and a specificity of 88.9%.

Conclusion: uNGAL and s-Cys-C predicts AKI early in critically ill children.

Keywords: Acute kidney injury; Neutrophil gelatinase-associated lipocalin; Serum cystatin C.

Publication types

  • Observational Study

MeSH terms

  • Acute Kidney Injury / diagnosis*
  • Acute Kidney Injury / epidemiology
  • Adolescent
  • Biomarkers / analysis*
  • Child
  • Child, Preschool
  • Cohort Studies
  • Cystatin C / blood*
  • Developing Countries
  • Early Diagnosis
  • Female
  • Humans
  • Incidence
  • Infant
  • Infant, Newborn
  • Intensive Care Units, Pediatric*
  • Lipocalin-2 / urine*
  • Male
  • Prospective Studies
  • Risk Assessment
  • Saudi Arabia / epidemiology
  • Sensitivity and Specificity
  • Severity of Illness Index

Substances

  • Biomarkers
  • Cystatin C
  • Lipocalin-2