Imaging the Lymph Node Stroma

Clément Ghigo; Rebecca Gentek; Marc Bajénoff

doi:10.1007/978-1-4939-7762-8_6

Imaging the Lymph Node Stroma

Methods Mol Biol. 2018:1763:53-61. doi: 10.1007/978-1-4939-7762-8_6.

Authors

Clément Ghigo¹, Rebecca Gentek¹, Marc Bajénoff²

Affiliations

¹ Aix-Marseille University, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunologie de Marseille-Luminy (CIML), Marseille, France.
² Aix-Marseille University, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunologie de Marseille-Luminy (CIML), Marseille, France. [email protected].

PMID: 29476488
DOI: 10.1007/978-1-4939-7762-8_6

Abstract

Lymph node (LN) stromal cells are being recognized as key organizers of the immune system. They assemble in complex 3D networks and hence, need to be studied in situ to fully understand their exact functions. Here, we describe two distinct but complementary procedures that allow analyzing LN stromal cells at high resolution by confocal imaging.

Keywords: 3D reconstruction; Confocal imaging; Cryostat sectioning; Lymph node; Stroma; Vibratome sectioning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Lymph Nodes / ultrastructure*
Microscopy, Confocal / methods*
Molecular Imaging / methods*
Stromal Cells / ultrastructure*