Using a functional proliferation reporter we identified quiescent tumor propagating cancer cells (TPCs) in intact squamous cell carcinomas, and found that TGFβ signaling controls their reversible entry into a growth arrested state, which protects TPCs from chemotherapy. TPCs with compromised TGFβ/Smad signaling can't enter quiescence and subsequently die from chemotherapy.
Keywords: TGFβ; cancer stem cells; cell cycle; chemotherapy; quiescence; resistance; skin; squamous cell carcinoma; tumor heterogeneity; tumor propagating cells.