Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone

Arch Virol. 2018 Jun;163(6):1677-1681. doi: 10.1007/s00705-018-3783-0. Epub 2018 Feb 27.

Abstract

The badnavirus taro bacilliform virus (TaBV) has been reported to infect taro (Colocasia esculenta L.) and other edible aroids in several South Pacific island countries, but there are no published reports from Australia. Using PCR and RCA, we identified and characterized an Australian TaBV isolate. A terminally redundant cloned copy of the TaBV genome was generated and shown to be infectious in taro following agro-inoculation. This is the first report of TaBV from Australia and also the first report of an infectious clone for this virus.

MeSH terms

  • Australia
  • Badnavirus / classification
  • Badnavirus / genetics*
  • Badnavirus / isolation & purification
  • Clone Cells
  • Colocasia / virology*
  • Genome, Viral*
  • Open Reading Frames*
  • Phylogeny
  • Plant Diseases / virology*
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA