Metabolism, Excretion, and Mass Balance of Solithromycin in Humans

Antimicrob Agents Chemother. 2018 Apr 26;62(5):e01474-17. doi: 10.1128/AAC.01474-17. Print 2018 May.

Abstract

Solithromycin, a novel macrolide and the first fluoroketolide, is being developed as a therapy for community-acquired bacterial pneumonia, with a distinct mechanism that provides activity against macrolide-resistant bacteria. The pharmacokinetics, metabolism, and excretion of solithromycin were studied in healthy male subjects after oral administration of a single 800-mg (∼100-μCi) dose of [14C]solithromycin. Solithromycin was well tolerated, and absorption from the solution occurred with a median time to peak concentration of 4.0 h. Solithromycin and the total radioactivity had similar profiles with no long-lived metabolites. The whole-blood total radioactivity was approximately 75% of the plasma total radioactivity. Recovery was essentially complete (mean, 90.6%), with 76.5% and 14.1% of the dose recovered in feces and urine, respectively. Unchanged solithromycin (CEM-101) was the predominant circulating radioactive component in plasma (77% of the total radioactivity area under the concentration-time curve [AUC]), with two minor plasma metabolites, CEM-214 and CEM-122 (N-acetyl-CEM-101), each accounting for approximately 5% of the total radioactivity. Urinary excretion was predominantly like that of the parent. Solithromycin was primarily eliminated in the feces after extensive metabolism via a complex metabolic pathway with CEM-262 as the major constituent (27.36% of the administered dose). Overall oxidative pathways, presumably carried out mostly by CYP3A4, represented the majority of the metabolism, with N-acetylation present to a lesser extent. No disproportionate human metabolites were observed.

Keywords: excretion; ketolide; macrolide; mass balance; metabolism; solithromycin.

MeSH terms

  • Adult
  • Anti-Bacterial Agents / metabolism*
  • Anti-Bacterial Agents / pharmacokinetics*
  • Community-Acquired Infections / drug therapy*
  • Cytochrome P-450 CYP3A / metabolism
  • Humans
  • Macrolides / metabolism*
  • Macrolides / pharmacokinetics*
  • Male
  • Metabolic Clearance Rate / physiology
  • Microbial Sensitivity Tests
  • Middle Aged
  • Pneumonia, Bacterial / drug therapy*
  • Triazoles / metabolism*
  • Triazoles / pharmacokinetics*
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Macrolides
  • Triazoles
  • solithromycin
  • Cytochrome P-450 CYP3A
  • CYP3A4 protein, human