Blocking the ART2.2/P2X7-system is essential to avoid a detrimental bias in functional CD4 T cell studies

Hristo Georgiev; Inga Ravens; Georgia Papadogianni; Bernard Malissen; Reinhold Förster; Günter Bernhardt

doi:10.1002/eji.201747420

Blocking the ART2.2/P2X7-system is essential to avoid a detrimental bias in functional CD4 T cell studies

Eur J Immunol. 2018 Jun;48(6):1078-1081. doi: 10.1002/eji.201747420. Epub 2018 Mar 23.

Authors

Hristo Georgiev¹, Inga Ravens¹, Georgia Papadogianni¹, Bernard Malissen², Reinhold Förster¹, Günter Bernhardt¹

Affiliations

¹ Institute of Immunology, Hannover Medical School, Hannover, Germany.
² Centre d'Immunologie de Marseille-Luminy, Marseille, France.

PMID: 29508376
DOI: 10.1002/eji.201747420

Abstract

Murine T cell subsets differ in their expression level of P2X7. Depending on several parameters like extracellular NAD⁺ , P2X7 can be ADP-ribosylated rapidly by adjacent ARTC2.2 resulting in susceptibilities to apoptosis to a varying extent. This detrimental effect can be prevented when drugs like KN-62 are present during cell preparations.

Keywords: ART2.2; Apoptosis; Follicular CD4 T cells; KN-62; P2X7; Regulatory T cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / analogs & derivatives
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / pharmacology
ADP Ribose Transferases / metabolism*
Adenosine Triphosphate / metabolism
Animals
Apoptosis
CD4-Positive T-Lymphocytes / immunology*
Cell Survival
Cells, Cultured
Ion Transport / drug effects*
Mice
Mice, Inbred C57BL
Mice, Transgenic
NAD / metabolism
Receptors, Purinergic P2X7 / genetics
Receptors, Purinergic P2X7 / metabolism*
T-Lymphocyte Subsets / immunology*

Substances

Receptors, Purinergic P2X7
NAD
KN 62
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Adenosine Triphosphate
ADP Ribose Transferases
Art2b protein, mouse