Tastant-Evoked Arc Expression in the Nucleus of the Solitary Tract and Nodose/Petrosal Ganglion of the Mouse Is Specific for Bitter Compounds

Chem Senses. 2018 Apr 23;43(4):261-272. doi: 10.1093/chemse/bjy017.

Abstract

Despite long and intense research, some fundamental questions regarding representation of taste information in the brain still remain unanswered. This might in part be due to shortcomings of the established methods that limit the researcher either to thorough characterization of few elements or to analyze the response of the entirety of neurons to only one stimulus. To overcome these restrictions, we evaluate the use of the immediate early gene Arc as a neuronal activity marker in the early neural structures of the taste pathway, the nodose/petrosal ganglion (NPG) and the nucleus of the solitary tract (NTS). Responses of NPG and NTS neurons were limited to substances that taste bitter to humans and are avoided by mice. Arc-expressing cells were concentrated in the rostromedial part of the dorsal NTS suggesting a role in gustatory processing. The use of Arc as a neuronal activity marker has several advantages, primarily the possibility to analyze the response of large numbers of neurons while using more than one stimulus makes Arc an interesting new tool for research in the early stages of taste processing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aversive Agents / pharmacology*
  • Brain Stem / metabolism
  • Brain Stem / pathology
  • Cytoskeletal Proteins / genetics
  • Cytoskeletal Proteins / metabolism*
  • Gene Expression Regulation / drug effects*
  • Image Processing, Computer-Assisted
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Nodose Ganglion / drug effects
  • Nodose Ganglion / metabolism*
  • Solitary Nucleus / drug effects
  • Solitary Nucleus / metabolism*
  • Sweetening Agents / pharmacology
  • Taste / physiology*

Substances

  • Aversive Agents
  • Cytoskeletal Proteins
  • Nerve Tissue Proteins
  • Sweetening Agents
  • activity regulated cytoskeletal-associated protein