A resumable two-photon fluorescent probe for Cu2+ and S2- based on magnetic silica core-shell Fe3O4@SiO2 nanoparticles and its application in bioimaging

Huie Jiang; Yan Liu; Weifang Luo; Yujiao Wang; Xiaoliang Tang; Wei Dou; Yumei Cui; Weisheng Liu

doi:10.1016/j.aca.2018.02.006

A resumable two-photon fluorescent probe for Cu²⁺ and S^2- based on magnetic silica core-shell Fe₃O₄@SiO₂ nanoparticles and its application in bioimaging

Anal Chim Acta. 2018 Jul 19:1014:91-99. doi: 10.1016/j.aca.2018.02.006. Epub 2018 Feb 10.

Authors

Huie Jiang¹, Yan Liu², Weifang Luo², Yujiao Wang², Xiaoliang Tang², Wei Dou², Yumei Cui², Weisheng Liu³

Affiliations

¹ Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China; College of Bioresources Chemistry and Materials Engineering, Shaanxi University of Science and Technology, Xi'an 710021, PR China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
² Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
³ Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic address: [email protected].

PMID: 29523257
DOI: 10.1016/j.aca.2018.02.006

Abstract

A two-photon fluorescent probe for Cu²⁺ and S^2- has been strategically prepared with naphthalimide derivative platform (NPE) covalently grafted onto the surface of magnetic core-shell Fe₃O₄@SiO₂ nanoparticles. The probe (NPE-Fe₃O₄@SiO₂) exhibits selective response to Cu²⁺ with enhanced fluorescence and efficient separation of Cu²⁺ with external magnetic field. The consequent product NPE-Fe₃O₄@SiO₂-Cu of NPE-Fe₃O₄@SiO₂ and Cu²⁺ can work as an excellent sensor for S^2- by removing Cu²⁺ from the complex with fluorescence decreased, recovering the fluorescence of the probe. Therefore, the constituted Off-On-Off type fluorescence monitoring system means the probe is resumable. Moreover, the probe has been used to quantitatively detect Cu²⁺ and S^2- with low detection limits, which are 0.28 μM and 0.12 μM, respectively. Furthermore, the probe shows low cytotoxicity and excellent membrane permeability, which has been successfully applied for monitoring Cu²⁺ and S^2- in living cells and imaging Cu²⁺ in deep-tissue with two-photon excited fluorescence.

Keywords: Bioimaging; Cu(2+); Fluorescent probe; Nanoparticles; S(2−); Two-photon.

MeSH terms

Copper / analysis*
Ferrosoferric Oxide / chemistry
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Magnetic Phenomena
Molecular Structure
Nanoparticles / chemistry*
Optical Imaging*
Particle Size
Photons*
Silicon Dioxide / chemistry
Sulfur / analysis*
Surface Properties
Tumor Cells, Cultured

Substances

Fluorescent Dyes
Sulfur
Silicon Dioxide
Copper
Ferrosoferric Oxide