Human sperm proteins identified by 2-dimensional electrophoresis and mass spectrometry and their relevance to a transcriptomic analysis

Karolina Nowicka-Bauer; Małgorzata Ozgo; Adam Lepczynski; Marzena Kamieniczna; Agnieszka Malcher; Wiesław Skrzypczak; Maciej Kurpisz

doi:10.1016/j.repbio.2018.02.003

Human sperm proteins identified by 2-dimensional electrophoresis and mass spectrometry and their relevance to a transcriptomic analysis

Reprod Biol. 2018 Jun;18(2):151-160. doi: 10.1016/j.repbio.2018.02.003. Epub 2018 Mar 8.

Authors

Karolina Nowicka-Bauer¹, Małgorzata Ozgo², Adam Lepczynski², Marzena Kamieniczna¹, Agnieszka Malcher¹, Wiesław Skrzypczak², Maciej Kurpisz³

Affiliations

¹ Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
² Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology, 26 Doktora Judyma Street, 71-466 Szczecin, Poland.
³ Institute of Human Genetics, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland. Electronic address: [email protected].

PMID: 29526398
DOI: 10.1016/j.repbio.2018.02.003

Abstract

The aim of this study was to identify and analyse human sperm proteins from normozoospermic men using 2-dimensional electrophoresis (2-DE) and mass spectrometry (MS). We identified 73 different sperm proteins, including two less characterized human sperm proteins, Annexin A7 (ANXA7) and c14orf105. Bioinformatic analysis of detected sperm proteins revealed new carbohydrate and lipid metabolic pathways, which supply energy to motile sperm. A comparison of our data with available mRNA microarray data from the human testis allows for validation of identified sperm proteins and aids in the recognition of their physiological pathways.

Keywords: Human sperm proteome; Metabolic pathways; Normozoospermic; Protein electrophoresis; Protein identification.

MeSH terms

Annexin A7 / metabolism
Electrophoresis, Gel, Two-Dimensional
Gene Expression Profiling / methods*
Humans
Male
Mass Spectrometry
Proteomics
Spermatozoa / metabolism*
Testis / metabolism*

Substances

Annexin A7