Application of ribonucleoside vanadyl complex (RVC) for developing a multifunctional tissue preservative solution

PLoS One. 2018 Mar 14;13(3):e0194393. doi: 10.1371/journal.pone.0194393. eCollection 2018.

Abstract

The quality of biological samples greatly affects the accuracy of scientific results. However, RNA in cryopreserved tissues gradually degrades during storage, leading to errors in the results of subsequent experiments. A suitable sample preservative solution can prolong storage and enhance the research value of samples. Here, we developed a sample preservative solution using the properties of the ribonucleoside vanadyl complex (RVC) and compared its effects on RNA and DNA quality, protein activity, and tissue morphology with the commercially available and widely used RNAlater® Stabilization Solution. The results showed that both the RVC-based preservative solution and RNAlater can effectively delay RNA degradation in tissue samples stored at 4°C or -80°C compared with samples stored without any preservative solution. In contrast to RNAlater, the RVC-based preservative solution did not result in damage to the tissue morphology or a loss of protein activity. Additionally, the RVC-based preservative solution did not affect the RNA and genomic DNA contents of the tissue samples or the results of subsequent experimental analyses. An RVC-based reagent can be used as a multifunctional yet relatively inexpensive tissue preservative solution to provide a comprehensive and cost-effective method for preserving samples for tissue banks.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Organ Preservation Solutions / chemistry*
  • Organ Preservation Solutions / pharmacology
  • Protein Stability / drug effects
  • RNA Stability / drug effects
  • Ribonucleosides / chemistry*
  • Ribonucleosides / pharmacology
  • Tissue Preservation / methods*
  • Vanadates / chemistry*
  • Vanadates / pharmacology

Substances

  • Organ Preservation Solutions
  • Ribonucleosides
  • vanadyl ribonucleoside complex
  • Vanadates

Grants and funding

This work was partially supported by the: Ministry of Science and Technology, Taiwan (grant MOST 105-2320-B-182A-004-) website: https://www.most.gov.tw/ Chang Gung Medical foundation, Taiwan (grant CRRPG3F0071). website: https://www.cgmh.org.tw/eng2002/index.asp The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.