The CLAVATA receptor FASCIATED EAR2 responds to distinct CLE peptides by signaling through two downstream effectors

Elife. 2018 Mar 15:7:e35673. doi: 10.7554/eLife.35673.

Abstract

Meristems contain groups of indeterminate stem cells, which are maintained by a feedback loop between CLAVATA (CLV) and WUSCHEL (WUS) signaling. CLV signaling involves the secretion of the CLV3 peptide and its perception by a number of Leucine-Rich-Repeat (LRR) receptors, including the receptor-like kinase CLV1 and the receptor-like protein CLV2 coupled with the CORYNE (CRN) pseudokinase. CLV2, and its maize ortholog FASCIATED EAR2 (FEA2) appear to function in signaling by CLV3 and several related CLV3/EMBRYO-SURROUNDING REGION (CLE) peptide ligands. Nevertheless, how signaling specificity is achieved remains unknown. Here we show that FEA2 transmits signaling from two distinct CLE peptides, the maize CLV3 ortholog ZmCLE7 and ZmFON2-LIKE CLE PROTEIN1 (ZmFCP1) through two different candidate downstream effectors, the alpha subunit of the maize heterotrimeric G protein COMPACT PLANT2 (CT2), and ZmCRN. Our data provide a novel framework to understand how diverse signaling peptides can activate different downstream pathways through common receptor proteins.

Keywords: CLAVATA signaling; Plant Development; Shoot Apical Meristem; maize; plant biology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis Proteins / genetics*
  • Gene Expression Regulation, Plant
  • Homeodomain Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / genetics
  • Ligands
  • Membrane Proteins / genetics*
  • Meristem / genetics*
  • Meristem / growth & development
  • Protein Serine-Threonine Kinases / genetics*
  • Receptors, Cell Surface / genetics
  • Signal Transduction / genetics
  • Zea mays / genetics*
  • Zea mays / growth & development

Substances

  • AT2G27250 protein, Arabidopsis
  • Arabidopsis Proteins
  • CLV2 protein, Arabidopsis
  • Homeodomain Proteins
  • Intracellular Signaling Peptides and Proteins
  • Ligands
  • Membrane Proteins
  • Receptors, Cell Surface
  • WUSCHEL protein, Arabidopsis
  • CLV1 protein, Arabidopsis
  • CORYNE protein, Arabidopsis
  • Protein Serine-Threonine Kinases

Grants and funding

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.