Abstract
Herein we report the design and synthesis of a series of simple phenol amide ERRγ agonists based on a hydrazone lead molecule. Our structure activity relationship studies in this series revealed the phenol portion of the molecule to be required for activity. Attempts to replace the hydrazone with more suitable chemotypes led to a simple amide as a viable alternative. Differential hydrogen-deuterium exchange experiments were used to help understand the structural basis for binding to ERRγ and aid in the development of more potent ligands.
Keywords:
Agonist; Amide; ERRγ; Nuclear receptor; Selective ligand.
Copyright © 2018 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Benzamides / chemical synthesis
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Benzamides / chemistry
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Benzamides / pharmacology*
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Binding Sites
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Drug Stability
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Estrogens / chemical synthesis
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Estrogens / chemistry
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Estrogens / pharmacology*
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HEK293 Cells
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Half-Life
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Humans
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Hydrazones / chemical synthesis
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Hydrazones / chemistry
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Hydrazones / pharmacology
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Microsomes, Liver / metabolism
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Molecular Structure
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Phenols / chemical synthesis
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Phenols / chemistry
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Phenols / pharmacology*
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Receptors, Estrogen / chemistry
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Receptors, Estrogen / metabolism*
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Structure-Activity Relationship
Substances
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Benzamides
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Estrogens
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Hydrazones
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Phenols
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Receptors, Estrogen
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estrogen receptor gamma