Technical note: A histochemical approach in diagnosing hanging mechanical asphyxia on cadavers undergoing advanced putrefactive phenomena

Med Leg J. 2018 Dec;86(4):208-213. doi: 10.1177/0025817218764754. Epub 2018 Mar 21.

Abstract

The authors used a particular protocol on putrefied corpses to highlight the cutaneous furrow. Two groups of cadavers were selected: in the first group (suicide by hanging mechanical asphyxia), the authors sampled cutaneous lozenges on the furrow still macroscopically recognisable, while in the second group (corpses deceased by different means), we sampled cutaneous lozenges in the hypostatic leaning. All specimens were divided in two: one underwent standard fixation while the other, previously rehydrated in Sandison's solution, was then fixed in formalin. All specimens were stained with hematoxylin and eosin and Resorcin-Fuchsin. Samples processed with formalin and the H&E staining underlined various artifacts; vice versa, the Sandison solution associated with Resorcin-Fuchsin staining demonstrated the compression of the elastic fibres, with focal positivity in hanged corpses, and diffuse positivity in the entire hypostatic leaning. Sandison's rehydrating solution and Resorcin-Fuchsin staining exalt the supposed application of an asphyctic mean even on putrefied corpses in those cases burdened by a diagnostic doubt.

Keywords: Forensic science; Resorcin-Fuchsin staining; Sandison; elastic fibres; putrefaction; rehydration.

MeSH terms

  • Asphyxia / pathology*
  • Cadaver
  • Case-Control Studies
  • Elastic Tissue / pathology
  • Forensic Pathology
  • Humans
  • Male
  • Middle Aged
  • Neck Injuries / pathology*
  • Postmortem Changes*
  • Rehydration Solutions
  • Staining and Labeling
  • Suicide, Completed

Substances

  • Rehydration Solutions