Abstract
A carbapenem-resistant Klebsiella pneumoniae isolate was recovered from human blood. Its whole-genome sequence was obtained using Illumina and long-read MinION sequencing. The strain belongs to sequence type 273 (ST273), which was found recently and caused an outbreak in Southeast Asia. It has two carbapenemase genes, blaNDM-1 (carried by an ST7 IncN self-transmissible plasmid) and blaIMP-4 (located on a self-transmissible IncHI5 plasmid). Non-KPC-producing ST237 may represent a lineage of carbapenem-resistant K. pneumoniae, which warrants further monitoring.
Keywords:
Klebsiella; Klebsiella pneumoniae; carbapenemases; carbapenems; plasmids; resistance; β-lactamases.
Copyright © 2018 American Society for Microbiology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics*
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Carbapenem-Resistant Enterobacteriaceae / genetics*
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Carbapenems / pharmacology*
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Disease Outbreaks
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Drug Resistance, Multiple, Bacterial / genetics*
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High-Throughput Nucleotide Sequencing
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Humans
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Klebsiella Infections / drug therapy
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Klebsiella pneumoniae / drug effects*
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Male
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Microbial Sensitivity Tests
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Pancreatitis / drug therapy
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Pancreatitis / microbiology
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Plasmids / genetics
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Whole Genome Sequencing
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beta-Lactamases / genetics*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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beta-lactamase IMP-4
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beta-Lactamases
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beta-lactamase NDM-1
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carbapenemase