Usefulness of Enhanced Liver Fibrosis, Glycosylation Isomer of Mac-2 Binding Protein, Galectin-3, and Soluble Suppression of Tumorigenicity 2 for Assessing Liver Fibrosis in Chronic Liver Diseases

Ann Lab Med. 2018 Jul;38(4):331-337. doi: 10.3343/alm.2018.38.4.331.

Abstract

Background: Liver biopsies have been partially replaced by noninvasive methods for assessing liver fibrosis. We explored the usefulness of four novel biomarkers, enhanced liver fibrosis (ELF), glycosylation isomer of Mac-2 binding protein (M2BPGi), galectin-3, and soluble suppression of tumorigenicity 2 (sST2), in association with liver fibrosis.

Methods: ELF, M2BPGi, galectin-3, and sST2 were assayed in 173 patients with chronic liver diseases. The results were analyzed according to fibrosis grade (F0/1, F2, and F3/4) by transient elastography (TE).

Results: ELF, M2BPGi, galectin-3, and sST2 values differed significantly according to TE grade; ELF and M2BPGi values were higher in F2 and F3/4 than in F0/1 (P≤0.001, all), sST2 values were higher in F3/4 than in F0/1 and F2 (P<0.05), and galectin-3 values were higher in F3/4 than in F0/1 (P=0.0036). ELF and M2BPGi showed good TE fibrosis detection performance (area under the curves [AUC], 0.841 and 0.833 for ≥F2; and 0.837 and 0.808 for ≥F3). The sensitivity and specificity for predicting TE grade F≥2 were 84.1% and 76.7% for ELF and 63.6% and 91.5% for M2BPGi.

Conclusions: This is the first study to compare the liver fibrosis assessment of four novel biomarkers: ELF, M2BPGi, galectin-3, and sST2. The biomarkers varied significantly according to TE grade, and each biomarker showed a different trend. ELF and M2BPGi seem to have comparable good performance for detecting liver fibrosis.

Keywords: Biomarker; ELF; Galectin-3; Liver fibrosis; M2BPGi; sST2.

MeSH terms

  • Adult
  • Aged
  • Antigens, Neoplasm / metabolism*
  • Area Under Curve
  • Biomarkers / metabolism
  • Blood Proteins
  • Chronic Disease
  • Elasticity Imaging Techniques
  • Female
  • Galectin 3 / metabolism*
  • Galectins
  • Glycosylation
  • Humans
  • Interleukin-1 Receptor-Like 1 Protein / metabolism*
  • Liver Cirrhosis / diagnosis*
  • Liver Cirrhosis / pathology
  • Liver Diseases / diagnosis*
  • Liver Diseases / pathology
  • Male
  • Membrane Glycoproteins / metabolism*
  • Middle Aged
  • ROC Curve
  • Sensitivity and Specificity
  • Severity of Illness Index

Substances

  • Antigens, Neoplasm
  • Biomarkers
  • Blood Proteins
  • Galectin 3
  • Galectins
  • IL1RL1 protein, human
  • Interleukin-1 Receptor-Like 1 Protein
  • LGALS3 protein, human
  • Membrane Glycoproteins
  • TAA90K protein, human