Development of a TaqMan-based real-time PCR assay for the rapid and specific detection of pigeon torque teno virus

Mol Cell Probes. 2018 Jun:39:53-56. doi: 10.1016/j.mcp.2018.04.001. Epub 2018 Apr 3.

Abstract

Pigeon torque teno virus (PTTV), a recently discovered circular DNA virus. Here, we developed a TaqMan-based real-time PCR for rapid and specific detection of PTTV infections with sensitivity up to 49.3 copies/μl. Positive signals can be observed by the assay in pigeon embryonated eggs, which indicted that PTTV can be transmitted vertically. Our findings play important implications for a better understanding the transmission of torque teno virus in pigeons.

Keywords: Pigeon torque teno virus; Real-time PCR; Vertical transmission.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Columbidae / virology*
  • Real-Time Polymerase Chain Reaction / methods*
  • Reference Standards
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Torque teno virus / isolation & purification*