Metabolite Depletion Affects Flux Profiling of Cell Lines

A Nilsson; J R Haanstra; B Teusink; J Nielsen

doi:10.1016/j.tibs.2018.03.009

Metabolite Depletion Affects Flux Profiling of Cell Lines

Trends Biochem Sci. 2018 Jun;43(6):395-397. doi: 10.1016/j.tibs.2018.03.009. Epub 2018 Apr 12.

Authors

A Nilsson¹, J R Haanstra², B Teusink², J Nielsen³

Affiliations

¹ Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg SE41296, Sweden.
² Systems Bioinformatics, Amsterdam Institute for Molecules, Medicines and Systems (AIMMS), Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands.
³ Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg SE41296, Sweden; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK2800 Kongens Lyngby, Denmark. Electronic address: [email protected].

PMID: 29656822
DOI: 10.1016/j.tibs.2018.03.009

Abstract

Quantifying the rate of consumption and release of metabolites (i.e., flux profiling) has become integral to the study of cancer. The fluxes as well as the growth of the cells may be affected by metabolite depletion during cultivation.

Keywords: cancer; glutamine; metabolic modeling; serine.

Publication types

Review

MeSH terms

Cell Culture Techniques
Humans
Neoplasms / metabolism*
Neoplasms / pathology*
Tumor Cells, Cultured