Membrane-containing virus particles exhibit the mechanics of a composite material for genome protection

Nanoscale. 2018 Apr 26;10(16):7769-7779. doi: 10.1039/c8nr00196k.

Abstract

The protection of the viral genome during extracellular transport is an absolute requirement for virus survival and replication. In addition to the almost universal proteinaceous capsids, certain viruses add a membrane layer that encloses their double-stranded (ds) DNA genome within the protein shell. Using the membrane-containing enterobacterial virus PRD1 as a prototype, and a combination of nanoindentation assays by atomic force microscopy and finite element modelling, we show that PRD1 provides a greater stability against mechanical stress than that achieved by the majority of dsDNA icosahedral viruses that lack a membrane. We propose that the combination of a stiff and brittle proteinaceous shell coupled with a soft and compliant membrane vesicle yields a tough composite nanomaterial well-suited to protect the viral DNA during extracellular transport.

MeSH terms

  • Bacteriophage PRD1 / genetics*
  • Capsid*
  • DNA, Viral / genetics*
  • Genome, Viral*
  • Microscopy, Atomic Force
  • Nanostructures
  • Virion

Substances

  • DNA, Viral