Combined Blockade of Interleukin-1α and -1β Signaling Protects Mice from Cognitive Dysfunction after Traumatic Brain Injury

eNeuro. 2018 Apr 13;5(2):ENEURO.0385-17.2018. doi: 10.1523/ENEURO.0385-17.2018. eCollection 2018 Mar-Apr.

Abstract

Diffuse activation of interleukin-1 inflammatory cytokine signaling after traumatic brain injury (TBI) elicits progressive neurodegeneration and neuropsychiatric dysfunction, and thus represents a potential opportunity for therapeutic intervention. Although interleukin (IL)-1α and IL-1β both activate the common type 1 IL-1 receptor (IL-1RI), they manifest distinct injury-specific roles in some models of neurodegeneration. Despite its potential relevance to treating patients with TBI, however, the individual contributions of IL-1α and IL-1β to TBI-pathology have not been previously investigated. To address this need, we applied genetic and pharmacologic approaches in mice to dissect the individual contributions of IL-1α, IL-β, and IL-1RI signaling to the pathophysiology of fluid percussion-mediated TBI, a model of mixed focal and diffuse TBI. IL-1RI ablation conferred a greater protective effect on brain cytokine expression and cognitive function after TBI than did individual IL-1α or IL-1β ablation. This protective effect was recapitulated by treatment with the drug anakinra, a recombinant naturally occurring IL-1RI antagonist. Our data thus suggest that broad targeting of IL-1RI signaling is more likely to reduce neuroinflammation and preserve cognitive function after TBI than are approaches that individually target IL-1α or IL-1β signaling.

Keywords: Inflammation; interleukin-1; traumatic brain injury.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal / drug effects
  • Brain Injuries, Traumatic* / complications
  • Brain Injuries, Traumatic* / immunology
  • Brain Injuries, Traumatic* / metabolism
  • Cognitive Dysfunction / etiology
  • Cognitive Dysfunction / immunology
  • Cognitive Dysfunction / metabolism
  • Cognitive Dysfunction / prevention & control*
  • Disease Models, Animal
  • Inflammation / etiology
  • Inflammation / immunology
  • Inflammation / metabolism
  • Inflammation / prevention & control*
  • Interleukin 1 Receptor Antagonist Protein / pharmacology
  • Interleukin-1alpha / antagonists & inhibitors
  • Interleukin-1alpha / deficiency
  • Interleukin-1alpha / metabolism*
  • Interleukin-1beta / antagonists & inhibitors
  • Interleukin-1beta / deficiency
  • Interleukin-1beta / metabolism*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Signal Transduction* / drug effects
  • Signal Transduction* / immunology

Substances

  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1alpha
  • Interleukin-1beta