Centrosome Inheritance Does Not Regulate Cell Fate in Granule Neuron Progenitors of the Developing Cerebellum

Cerebellum. 2018 Oct;17(5):685-691. doi: 10.1007/s12311-018-0935-4.

Abstract

An inherent asymmetry exists between the two centrosomes of a dividing cell. One centrosome is structurally more mature (mother centrosome) than the other (daughter centrosome). Post division, one daughter cell inherits the mother centrosome while the other daughter cell inherits the daughter centrosome. Remarkably, the kind of centrosome inherited is associated with cell fate in several developmental contexts such as in radial glial progenitors in the developing mouse cortex, Drosophila neuroblast divisions and in Drosophila male germline stem cells. However, the role of centrosome inheritance in granule neuron progenitors in the developing cerebellum has not been investigated. Here, we show that mother and daughter centrosomes do exist in these progenitors, and the amount of pericentriolar material (PCM) each centrosome possesses is different. However, we failed to observe any correlation between the fate adopted by the daughter cell and the nature of centrosome it inherited.

Keywords: Cell fate; Cerebellar granule neuron progenitors (CGNPs); Mother centrosome; Pericentriolar material (PCM).

MeSH terms

  • Animals
  • Brain Stem / cytology
  • Brain Stem / growth & development
  • Brain Stem / metabolism
  • Cells, Cultured
  • Centrosome / physiology*
  • Cerebellum / cytology
  • Cerebellum / growth & development*
  • Cerebellum / metabolism
  • Heat-Shock Proteins / metabolism
  • Immunohistochemistry
  • Mesencephalon / cytology
  • Mesencephalon / growth & development
  • Mesencephalon / metabolism
  • Mice
  • Mitosis / physiology
  • Neural Stem Cells / physiology*
  • Neurons / physiology*

Substances

  • Heat-Shock Proteins
  • Odf2 protein, mouse