H2AFY is a novel fusion partner of MECOM in acute myeloid leukemia

Qiaoyan Han; Jiao Lu; Jianjiang Wang; Jinsong Ye; Xin Jiang; Haoyue Chen; Chunhua Liu; Lu Chen; Tong Lin; Suning Chen; Miao Sun; Feng Gao

doi:10.1016/j.cancergen.2018.01.004

H2AFY is a novel fusion partner of MECOM in acute myeloid leukemia

Cancer Genet. 2018 Apr:222-223:9-12. doi: 10.1016/j.cancergen.2018.01.004. Epub 2018 Feb 17.

Authors

Qiaoyan Han¹, Jiao Lu², Jianjiang Wang³, Jinsong Ye⁴, Xin Jiang⁵, Haoyue Chen⁶, Chunhua Liu⁷, Lu Chen⁸, Tong Lin⁹, Suning Chen¹⁰, Miao Sun¹¹, Feng Gao¹²

Affiliations

¹ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
² Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
³ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
⁴ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
⁵ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
⁶ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
⁷ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
⁸ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
⁹ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
¹⁰ Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. Electronic address: [email protected].
¹¹ Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].
¹² Department of Haematology, Jingjiang People's Hospital, the Seventh Affiliated Hospital of Yangzhou University, Jingjiang, Jiangsu, China. Electronic address: [email protected].

PMID: 29666008
DOI: 10.1016/j.cancergen.2018.01.004

Abstract

The MECOM gene encoding a zinc finger protein that functions as a transcription factor, was located on chromosome 3q26, and rearrangements of MECOM often cause its overexpression in acute myeloid leukemia (AML). We identified H2AFY as a novel fusion gene partner of MECOM in an elderly male AML patient with cryptic 3q26 rearrangement using the whole transcriptome sequencing, who carried out abnormal karyotype of 46,XY,t(3;5)(q27;q31),add(14)(p11). We validated the existence of the unreported H2AFY-MECOM fusion gene by RT-PCR and Sanger DNA sequencing, and detected mutations of NRAS and BCOR in this patient. In addition, we found abnormally elevated expression of MECOM in this patient by quantitative-polymerase chain reaction (RQ-PCR). Further research is needed to investigate functional characterizations of this novel fusion in the development of AML.

Keywords: Acute myeloid leukemia; H2AFY; MECOM.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Aged
Antineoplastic Combined Chemotherapy Protocols / therapeutic use
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 5
Fatal Outcome
Gene Rearrangement
Granulocyte Colony-Stimulating Factor / therapeutic use
Histones / genetics*
Humans
Induction Chemotherapy
Karyotyping
Leukemia, Myeloid, Acute / drug therapy
Leukemia, Myeloid, Acute / genetics*
MDS1 and EVI1 Complex Locus Protein / genetics*
Male
Oncogene Proteins, Fusion / genetics*
Real-Time Polymerase Chain Reaction
Sequence Analysis, DNA
Translocation, Genetic

Substances

Histones
MACROH2A2 protein, human
MDS1 and EVI1 Complex Locus Protein
MECOM protein, human
Oncogene Proteins, Fusion
Granulocyte Colony-Stimulating Factor