Generation of induced pluripotent stem cells from a patient with X-linked juvenile retinoschisis

Chi-Hsien Peng; Kang-Chieh Huang; Huai-En Lu; Shih-Han Syu; Aliaksandr A Yarmishyn; Jyh-Feng Lu; Waradee Buddhakosai; Tai-Chi Lin; Chih-Chien Hsu; De-Kuang Hwang; Chia-Ning Shen; Shih-Jen Chen; Shih-Hwa Chiou

doi:10.1016/j.scr.2018.04.005

Generation of induced pluripotent stem cells from a patient with X-linked juvenile retinoschisis

Stem Cell Res. 2018 May:29:152-156. doi: 10.1016/j.scr.2018.04.005. Epub 2018 Apr 11.

Authors

Affiliations

¹ Department of Ophthalmology, Shin Kong Wu Ho-Su Memorial Hospital, Fu-Jen Catholic University, Taipei, Taiwan.
² Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
³ Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan.
⁴ Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
⁵ School of Medicine, Fu Jen Catholic University, Taipei, Taiwan.
⁶ Institute of Clinical Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan.
⁷ Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
⁸ Genomic Research Center, Academia Sinica, Taipei, Taiwan.
⁹ Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan; Genomic Research Center, Academia Sinica, Taipei, Taiwan; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan. Electronic address: [email protected].

PMID: 29674172
DOI: 10.1016/j.scr.2018.04.005

Abstract

X-linked juvenile retinoschisis (XLRS) is a hereditary retinal dystrophy manifested as splitting of anatomical layers of retina. In this report, we generated a patient-specific induced pluripotent stem cell (iPSC) line, TVGH-iPSC-013-05, from the peripheral blood mononuclear cells of a male patient with XLRS by using the Sendai-virus delivery system. We believe that XLRS patient-specific iPSCs provide a powerful in vitro model for evaluating the pathological phenotypes of the disease.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Cell Line
Cellular Reprogramming Techniques*
Humans
Induced Pluripotent Stem Cells* / metabolism
Induced Pluripotent Stem Cells* / pathology
Male
Retinoschisis* / genetics
Retinoschisis* / metabolism
Retinoschisis* / pathology