Development of an HPLC/UV method for the evaluation of extractables and leachables in plastic: Application to a plastic-packaged calcium gluconate glucoheptonate solution

J Pharm Biomed Anal. 2018 Jun 5:155:298-305. doi: 10.1016/j.jpba.2018.03.044. Epub 2018 Mar 21.

Abstract

Calcium gluconate glucoheptonate (GGCa) is known to interact with glass containers, leading to the leaching of aluminum from the glass into the solution at toxic level. Therefore, plastic containers seem to be a preferable packaging alternative. Nevertheless, plastics contain potentially toxic additives which could be released into the solution. In order to study content container interaction between GGCa and two plastic containers (polypropylene PP and polyethylene PE containers), an HPLC-PDA method was developed to separate, detect and quantify eleven additives commonly found in plastic materials, with good limit of detection and quantification. This method was then applied to evaluate the compatibility between GGCa and the two plastic containers. After 3 months of storage at 25 °C, none of the eleven additives were detected in GGCa solutions. The safety concern threshold (SCT) and of the analytical evaluation threshold (AET) were evaluated to discriminate the need to identify and qualify unknown peaks.

Keywords: Analytical evaluation threshold; Calcium gluconate glucoheptonate; Content container interaction; Extractable; Leachable; Safety concern threshold.

MeSH terms

  • Calcium Gluconate / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Drug Contamination / prevention & control
  • Drug Packaging / methods
  • Glass / analysis
  • Glass / chemistry
  • Gluconates / chemistry*
  • Plastics / analysis*
  • Plastics / chemistry*
  • Polypropylenes / chemistry
  • Sugar Acids / chemistry*
  • Ultraviolet Rays

Substances

  • Gluconates
  • Plastics
  • Polypropylenes
  • Sugar Acids
  • alpha-glucoheptonic acid
  • gluconic acid
  • Calcium Gluconate