Nanoparticle-Mediated Delivery towards Advancing Plant Genetic Engineering

Trends Biotechnol. 2018 Sep;36(9):882-897. doi: 10.1016/j.tibtech.2018.03.009. Epub 2018 Apr 24.

Abstract

Genetic engineering of plants has enhanced crop productivity in the face of climate change and a growing global population by conferring desirable genetic traits to agricultural crops. Efficient genetic transformation in plants remains a challenge due to the cell wall, a barrier to exogenous biomolecule delivery. Conventional delivery methods are inefficient, damaging to tissue, or are only effective in a limited number of plant species. Nanoparticles are promising materials for biomolecule delivery, owing to their ability to traverse plant cell walls without external force and highly tunable physicochemical properties for diverse cargo conjugation and broad host range applicability. With the advent of engineered nuclease biotechnologies, we discuss the potential of nanoparticles as an optimal platform to deliver biomolecules to plants for genetic engineering.

Keywords: biomolecule delivery; gene editing; nanoparticle; plants.

Publication types

  • Review

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Biolistics / instrumentation
  • Biolistics / methods
  • Cell Wall / chemistry
  • Cell Wall / metabolism
  • Crops, Agricultural / genetics*
  • Crops, Agricultural / growth & development
  • Electroporation / instrumentation
  • Electroporation / methods
  • Gene Editing / methods*
  • Genetic Engineering / methods*
  • Genome, Plant
  • Government Regulation
  • Humans
  • Microinjections / instrumentation
  • Microinjections / methods
  • Nanoparticles / chemistry
  • Nanoparticles / metabolism*
  • Plant Cells / chemistry
  • Plant Cells / metabolism
  • Plants, Genetically Modified*
  • Transformation, Genetic
  • Transgenes