Objective: To investigate the relationship between the serum anti-Müllerian hormone (AMH) level and semen parameters.
Methods: We collected the data about 726 outpatients at the Male Infertility Clinic of Jinling Hospital from September 2015 to November 2016, including 72 with non-obstructive azoospermia, 123 with oligospermia, and 531 with normal sperm concentration. We obtained the semen volume, total sperm count, sperm concentration, sperm motility, the percentages of progressively motile sperm (PMS) and morphologically normal sperm (MNS), and the levels of serum AMH, inhibin B (INH-B), total testosterone (TT) and follicle - stimulating hormone (FSH) of the patients, analyzed the correlation of the serum AMH level with the other parameters, and compared the AMH level among different groups.
Results: The serum AMH level was found to be correlated positively with the total sperm count (r = 0.227, P <0.001), sperm concentration (r = 0.215, P <0.001), sperm motility (r = 0.111, P = 0.003), the percentage of PMS (r = 0.120, P = 0.001), and the levels of INH-B (r = 0.399, P <0.001) and TT (r = 0.184, P = 0.002), negatively with the FSH level (r = －0.283, P <0.001), but insignificantly with age, time of abstinence, semen volume, and the percentage of MNS (P >0.05). There was a statistically significant difference in the serum AMH level among the patients with non-obstructive azoospermia, oligozoospermia, and normal sperm concentration (［6.33 ± 4.26］ vs ［8.26 ± 3.98］ vs ［9.8 ± 5.19］ ng/ml, P <0.001).
Conclusions: Serum AMH is a biomarker reflecting the function of Sertoli cells and its level is significantly correlated with sperm concentration and motility, suggesting that AMH may be involved in spermatogenesis and sperm maturation.
目的： 本研究旨在探讨血清抗苗勒管激素（AMH）与精液参数之间的关系。方法： 收集我院2015年9月至2016年11月男性不育门诊就诊患者的资料共计726例，其中非梗阻性无精子症者72例，少精子症者123例，正常精子浓度者531例。对患者进行精液常规和血清学检测，包括精液量、精子总数、精子浓度、精子活动率、前向运动精子百分率率、正常形态精子百分率和AMH、抑制素B（Inh-B）、睾酮（T）、卵泡刺激素（FSH），整理数据进行回顾性分析。结果： 血清AMH水平与精子总数（r=0.227，P<0.001）、精子浓度（r=0.215，P<0.001）、精子活动率（r=0.111，P=0.003）、前向运动精子百分率（r=0.120，P=0.001）、Inh-B（r=0.399，P<0.001）、T（r=0.184，P=0.002）之间存在一定的正相关；与FSH（r=－0.283，P<0.001）存在负相关；与年龄、禁欲时间、精液量和正常形态精子百分率之间（P>0.05）均无显著相关性。非梗阻性无精子症组、少精子症组和正常组患者之间血清AMH水平有显著差异（6.33±4.26 vs 8.26±3.98 vs 9.8±5.19 ng/ml，P<0.001）。结论： AMH是反映睾丸支持细胞功能的一个生物标志物；血清AMH水平与精子浓度和活动率明显相关，提示AMH可能与精子的发生和成熟有关。.