Objective: To investigate the expressions of interleukin-17 (IL-17) and interleukin-8 (IL-8) in benign prostatic hyperplasia (BPH) and BPH complicated with histological inflammation and their significance.
Methods: According to the results of HE staining, we divided 60 cases of BPH treated by transurethral resection of the prostate (TURP) into a BPH group (n = 23) and a BPH with inflammation group (n = 37). We analyzed the clinical data of the patients and determined the mRNA and protein expressions of IL-17 and IL-8 by immunohistochemistry, real-time fluorescence quantitative PCR, and Western blot, respectively.
Results: Compared with the BPH patients complicated with inflammation, the BPH group showed significantly lower International Prostate Symptom Score (IPSS) (29.1 ± 6.2 vs 21.6 ± 3.7), quality of life score (QoL) (5.4 ± 1.3 vs 4.4 ± 1.6), postvoid residual urine volume (RUV) (［198.6 ± 15.5］ vs ［98.2 ± 19.3］ ml), prostate volume (［69.2 ± 24.1］ vs ［49.8 ± 16.5］ ml), PSA level (［7.4 ± 1.9］ vs ［2.8 ± 0.8］ μg/L) and serum c-reactive protein content (CRP) (［5.1±2.0］ vs ［1.5±0.6］ mg/L), but a higher maximum urine flow rate (Qmax) (［4.7 ± 2.1］ vs ［8.2 ± 1.8］ ml/s) (all P<0.05). The former group had a significantly higher incidence rate of urinary retention than the latter (32.4% ［12/37］ vs 8.69% ［2/23］), mRNA expressions of IL-17 (0.303 ± 0.076 vs 0.042 ± 0.019) and IL-8 (0.536 ± 0.059 vs 0.108 ± 0.025), and protein expressions of IL-17 (0.88 ± 0.10 vs 0.34 ± 0.05) and IL-8 (1.07 ± 0.08 vs 0.43 ± 0.04) (all P<0.05).
Conclusions: The expressions of IL-17 and IL-8 are upregulated in the prostatic tissue of the BPH patients with inflammation, which may play a significant role in the development and progression of BPH.
目的： 探讨单纯良性前列腺增生（BPH）与伴有前列腺炎的BPH患者前列腺组织中IL-17和 IL-8表达差异。方法： 收集福建医科大学附属第一医院行经尿道前列腺电切术的60例BPH患者标本。根据标本HE染色不同分为单纯BPH组23例和合并炎症的BPH组37例。对两组患者的临床资料进行分析比较；用免疫组织化学方法检测各标本IL-17和IL-8的表达情况，用荧光定量PCR和Western印迹检测各标本IL-17和IL-8 mRNA和蛋白的表达。结果： 单纯BPH组和合并炎症的BPH组患者的国际前列腺症状评分（IPSS）［（21.6±3.7）分 vs （29.1±6.2）分］、生活质量评分（QoL）［（4.4±1.6）分 vs (5.4±1.3) 分］、最大尿流率（Qmax）［(8.2±1.8) ml/s vs (4.7±2.1) ml/s］、残余尿量（RUV）［(98.2±19.3) ml vs (198.6±15.5) ml］、前列腺体积［(49.8±16.5) ml vs (69.2±24.1) ml］、PSA［(2.8±0.8) μg/L vs (7.4±1.9) μg/L］和C反应蛋白（CRP）［(1.5±0.6) mg/L vs (5.1±2.0) mg/L］,两组相比差异明显，有统计学意义（P<0.05）；合并炎症的BPH组患者尿潴留的发生率［32.4%（12/37）］明显高于单纯BPH组［8.69%（2/23），P<0.05］；IL-17 mRNA和IL-8 mRNA在合并炎症的BPH组前列腺组织中的表达量(0.303±0.076和0.536±0.059)均明显高于单纯BPH组（0.042±0.019和0.108±0.025，P<0.05）；IL-17和IL-8蛋白在单纯BPH组和在合并炎症的BPH组患者前列腺组织中的蛋白表达(0.34±0.05 vs 0.88±0.10、0.43±0.04 vs 1.07±0.08)，两者相比差异显著，有统计学意义（P<0.05）。结论：IL-17和IL-8在合并炎症的BPH患者前列腺组织中的表达上调，其可能在BPH的发生发展中起重要作用。.
Keywords: benign prostatic hyperplasia; inflammation; interleukin-17; interleukin-8.