Antibodies and methods for immunohistochemistry of extracellular matrix proteins

Steffen Rickelt; Richard O Hynes

doi:10.1016/j.matbio.2018.04.011

Antibodies and methods for immunohistochemistry of extracellular matrix proteins

Matrix Biol. 2018 Oct:71-72:10-27. doi: 10.1016/j.matbio.2018.04.011. Epub 2018 May 3.

Authors

Steffen Rickelt¹, Richard O Hynes²

Affiliations

¹ David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: [email protected].
² David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Electronic address: [email protected].

PMID: 29730502
DOI: 10.1016/j.matbio.2018.04.011

Abstract

The diversity of extracellular matrix (ECM) proteins encoded in mammalian genomes and detected by proteomic analyses generates a need for well validated antibodies against these proteins. We present characterization of a large number of antibodies against ECM proteins, from both commercial and academic sources, together with discussion of methods and strategies for their effective use in immunohistochemistry and illustrations of their efficacy. These data should be of value to investigators seeking well validated antibodies to ECM proteins of interest and save significant time and money tracking down effective reagents.

Keywords: Antibody validation; Extracellular matrix antibodies; Immunohistochemistry.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Antibodies / metabolism*
Drug Industry
Extracellular Matrix / metabolism
Extracellular Matrix Proteins / immunology*
Humans
Immunohistochemistry

Substances

Antibodies
Extracellular Matrix Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding