Intermittent streptozotocin administration induces behavioral and pathological features relevant to Alzheimer's disease and vascular dementia

Neuropharmacology. 2018 Jul 15:137:164-177. doi: 10.1016/j.neuropharm.2018.04.021. Epub 2018 May 5.

Abstract

Rationale: Diabetes mellitus (DM) is a major risk factor for Alzheimer's disease and vascular dementia. Few animal models exist that focus on the metabolic contributions to dementia onset and progression. Thus, there is strong scientific rationale to explore the effects of streptozotocin (STZ), a diabetogenic compound, on vascular and inflammatory changes within the brain.

Objective and methods: The present study was designed to evaluate the effect of staggered, low-dose administration of STZ on behavioral and cognitive deficits, neuroinflammation, tau pathology, and histopathological alterations related to dementia.

Results: Staggered administration (Days 1, 2, 3, 14, 15) of streptozotocin (40 mg/kg/mL) induced a diabetic-like state in mice, resulting in sustained hyperglycemia. STZ-treated animals displayed memory deficits in the novel object recognition task as well as increased tau phosphorylation and increased neuroinflammation. Additionally, STZ led to altered insulin signaling, exhibited by decreased plasma insulin and decreased levels of insulin degrading enzyme and pAKT within the hippocampus.

Conclusions: STZ-treated animals exhibit cognitive deficits and histopathological changes seen in dementia. This model of dementia warrants continued investigation to better understand the role that DM plays in dementia-related alterations.

Keywords: Alzheimer's disease; Diabetes mellitus; Insulin; Neuroinflammation; Streptozotocin; Streptozotocin (PubChem CID: 5300); Vascular dementia.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alzheimer Disease / etiology*
  • Alzheimer Disease / metabolism
  • Alzheimer Disease / pathology
  • Alzheimer Disease / psychology
  • Animals
  • Brain / blood supply
  • Brain / immunology
  • Brain / pathology
  • Dementia, Vascular / etiology*
  • Dementia, Vascular / metabolism
  • Dementia, Vascular / pathology
  • Dementia, Vascular / psychology
  • Diabetes Mellitus, Experimental / complications*
  • Diabetes Mellitus, Experimental / metabolism
  • Diabetes Mellitus, Experimental / pathology
  • Diabetes Mellitus, Experimental / psychology
  • Hemorrhage / pathology
  • Hyperglycemia / metabolism
  • Hyperglycemia / pathology
  • Hyperglycemia / psychology
  • Inflammation / etiology
  • Inflammation / metabolism
  • Inflammation / pathology
  • Inflammation / psychology
  • Insulin / metabolism
  • Learning Disabilities / metabolism
  • Learning Disabilities / pathology
  • Male
  • Memory Disorders / metabolism
  • Memory Disorders / pathology
  • Mice, Inbred C57BL
  • Microvessels / pathology
  • Streptozocin / administration & dosage
  • tau Proteins / metabolism

Substances

  • Insulin
  • Mapt protein, mouse
  • tau Proteins
  • Streptozocin