Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia

Séverine Nobis; Alexis Goichon; Najate Achamrah; Charlène Guérin; Saida Azhar; Philippe Chan; Aline Morin; Christine Bôle-Feysot; Jean Claude do Rego; David Vaudry; Pierre Déchelotte; Liliana Belmonte; Moïse Coëffier

doi:10.1038/s41598-018-25548-9

Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia

Sci Rep. 2018 May 8;8(1):7233. doi: 10.1038/s41598-018-25548-9.

Authors

Séverine Nobis^{1

2}, Alexis Goichon^{1

2}, Najate Achamrah^{1

2

3}, Charlène Guérin^{1

2}, Saida Azhar^{1

2}, Philippe Chan^{2

4}, Aline Morin^{1

2}, Christine Bôle-Feysot^{1

2}, Jean Claude do Rego^{2

5}, David Vaudry^{2

4

6}, Pierre Déchelotte^{1

2

3}, Liliana Belmonte^{1

2

3}, Moïse Coëffier^{7

8

9}

Affiliations

¹ Normandie University, UNIROUEN, INSERM Unit 1073, Rouen, France.
² Normandie University, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France.
³ Rouen University Hospital, Nutrition Department, Rouen, France.
⁴ Normandie University, UNIROUEN, Platform in proteomics PISSARO, Rouen, France.
⁵ Normandie University, UNIROUEN, Animal Behaviour Platform SCAC, Rouen, France.
⁶ Normandie University, UNIROUEN, INSERM Unit 1239, Mont-Saint-Aignan, France.
⁷ Normandie University, UNIROUEN, INSERM Unit 1073, Rouen, France. [email protected].
⁸ Normandie University, UNIROUEN, Institute for Research and Innovation in Biomedicine (IRIB), Rouen, France. [email protected].
⁹ Rouen University Hospital, Nutrition Department, Rouen, France. [email protected].

Abstract

Restrictive anorexia nervosa is associated with reduced eating and severe body weight loss leading to a cachectic state. Hypothalamus plays a major role in the regulation of food intake and energy homeostasis. In the present study, alterations of hypothalamic proteome and particularly of proteins involved in energy and mitochondrial metabolism have been observed in female activity-based anorexia (ABA) mice that exhibited a reduced food intake and a severe weight loss. In the hypothalamus, mitochondrial dynamic was also modified during ABA with an increase of fission without modification of fusion. In addition, increased dynamin-1, and LC3II/LC3I ratio signed an activation of autophagy while protein synthesis was increased. In conclusion, proteomic analysis revealed an adaptive hypothalamic protein response in ABA female mice with both altered mitochondrial response and activated autophagy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aconitate Hydratase / genetics
Aconitate Hydratase / metabolism
Animals
Anorexia
Anorexia Nervosa / genetics*
Anorexia Nervosa / metabolism
Anorexia Nervosa / physiopathology
Autophagy / genetics
Disease Models, Animal
Dynamin I / genetics*
Dynamin I / metabolism
Eating / genetics
Female
Gene Expression Profiling
Gene Expression Regulation
Hypothalamus / metabolism*
Hypothalamus / physiopathology
Mice
Mice, Inbred C57BL
Microtubule-Associated Proteins / genetics*
Microtubule-Associated Proteins / metabolism
Mitochondrial Dynamics / genetics*
Physical Conditioning, Animal
Protein Biosynthesis
Protein Isoforms / genetics
Protein Isoforms / metabolism
Proteome / genetics*
Proteome / metabolism
Receptors, Enterotoxin / genetics
Receptors, Enterotoxin / metabolism
Signal Transduction
Weight Loss / genetics

Substances

Map1lc3b protein, mouse
Microtubule-Associated Proteins
Protein Isoforms
Proteome
Dynamin I
Aconitate Hydratase
aconitase 2, mouse
Gucy2c protein, mouse
Receptors, Enterotoxin