Studies on the hepatoprotective effect of fucoidans from brown algae Kjellmaniella crassifolia

Shu Liu; Qiukuan Wang; Yuefan Song; Yunhai He; Dandan Ren; Haihua Cong; Long Wu

doi:10.1016/j.carbpol.2018.03.077

Studies on the hepatoprotective effect of fucoidans from brown algae Kjellmaniella crassifolia

Carbohydr Polym. 2018 Aug 1:193:298-306. doi: 10.1016/j.carbpol.2018.03.077. Epub 2018 Mar 23.

Authors

Shu Liu¹, Qiukuan Wang², Yuefan Song³, Yunhai He⁴, Dandan Ren⁵, Haihua Cong⁶, Long Wu⁷

Affiliations

¹ Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: [email protected].
² Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: [email protected].
³ Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: [email protected].
⁴ Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: [email protected].
⁵ Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: [email protected].
⁶ Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: [email protected].
⁷ Key Laboratory of Aquatic Products Processing and Utilization of Liaoning Province, National R & D Branch Center for Seaweed Processing, College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, PR China. Electronic address: [email protected].

PMID: 29773385
DOI: 10.1016/j.carbpol.2018.03.077

Abstract

For illustrating the relation of different structural characteristics correlated with the hepatoprotective effect, studies of fucoidan from algae Kjellmaniella crassifolia, a brown alga distributed in north Japan, were carried out. The fucoidan fractions of K. crassifolia were extracted, separated, and purified using a combinatorial procedure consisting of enzymolysis, ethanol precipitation, DEAE and size-exclusion chromatographies. The fundamental characteristics of the four enriched fucoidan fractions (KF1-KF4), including their sulphate content and monosaccharide composition, were investigated. The fucose was the main composition of monosaccharide for KF1-KF4, that of KF4 was up to 91.4%. The Glu-UA was the special composition of monosaccharide. FTIR and NMR spectroscopy were employed to elucidate the structural features of all fractions which further illustrated that fucose was the main monosaccharide. It was found all the four fractions showed antioxidative activity against hydroxyl radical and had the bioactive effects on CCl₄-induced liver injury.

Keywords: Fucoidan; Kjellmaniella crassifolia; Liver injury; Structural characterization.