A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield

M Furutani; M Iwaki; T Yagi; M Iida; K Horiike; M Nozaki

doi:10.1016/0020-711x(88)90238-8

A simple purification method for a glucosyltransferase complex from Streptococcus mutans OMZ 176 with a high yield

Int J Biochem. 1988;20(11):1327-32. doi: 10.1016/0020-711x(88)90238-8.

Authors

M Furutani¹, M Iwaki, T Yagi, M Iida, K Horiike, M Nozaki

Affiliation

¹ Department of Oral and Maxillofacial Surgery, Shiga University of Medical Science, Japan.

PMID: 2977765
DOI: 10.1016/0020-711x(88)90238-8

Abstract

1. Glucosyltransferase was purified from Streptococcus mutans OMZ 176 by ammonium sulfate fractionation and gel filtration. 2. The overall purification was about 15,000-fold with a yield of 53%. 3. The purified enzyme appeared to be a multienzyme complex consisting of three different components with molecular weights of 158,000, 153,000 and 149,000, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ammonium Sulfate
Chromatography / methods
Chromatography, Gel
Glucosyltransferases / isolation & purification*
Molecular Weight
Polysaccharides, Bacterial / isolation & purification
Streptococcus / enzymology*

Substances

Polysaccharides, Bacterial
Glucosyltransferases
Ammonium Sulfate