Abstract
Plant resistance genes typically encode proteins with nucleotide binding site-leucine rich repeat (NLR) domains. Here we show that Ptr is an atypical resistance gene encoding a protein with four Armadillo repeats. Ptr is required for broad-spectrum blast resistance mediated by the NLR R gene Pi-ta and by the associated R gene Pi-ta2. Ptr is expressed constitutively and encodes two isoforms that are mainly localized in the cytoplasm. A two base pair deletion within the Ptr coding region in the fast neutron-generated mutant line M2354 creates a truncated protein, resulting in susceptibility to M. oryzae. Targeted mutation of Ptr in a resistant cultivar using CRISPR/Cas9 leads to blast susceptibility, further confirming its resistance function. The cloning of Ptr may aid in the development of broad spectrum blast resistant rice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Armadillo Domain Proteins / genetics*
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Armadillo Domain Proteins / immunology
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CRISPR-Cas Systems
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Chromosome Mapping
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Disease Resistance / genetics*
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Genes, Plant / immunology*
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Host Microbial Interactions / genetics
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Host Microbial Interactions / immunology
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Magnaporthe / immunology
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Magnaporthe / pathogenicity
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Mutagenesis
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Oryza / microbiology
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Oryza / physiology*
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Plant Diseases / genetics*
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Plant Diseases / microbiology
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Plant Immunity / genetics
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Plant Proteins / genetics*
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Plant Proteins / immunology
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Plants, Genetically Modified
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Protein Isoforms / genetics
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Protein Isoforms / immunology
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Receptors, Cytoplasmic and Nuclear / genetics*
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Receptors, Cytoplasmic and Nuclear / immunology
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Sequence Analysis, DNA
Substances
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Armadillo Domain Proteins
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Pi-ta protein, Oryza sativa
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Plant Proteins
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Protein Isoforms
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Receptors, Cytoplasmic and Nuclear