Effects of different periodontal treatments in changing the prevalence and levels of Archaea present in the subgingival biofilm of subjects with periodontitis: A secondary analysis from a randomized controlled clinical trial

Int J Dent Hyg. 2018 Nov;16(4):569-575. doi: 10.1111/idh.12347. Epub 2018 May 23.

Abstract

Objective: The aim of this randomized double-blind and placebo-controlled study was to assess if periodontal treatment with or without systemic antibiotic would change the mean level of Archaea.

Methods: Fifty-nine (59) subjects were randomly assigned to receive scaling and root planing (SRP) alone or combined with metronidazole (MTZ; 400 mg/TID) or either with MTZ and amoxicillin (AMX; 500 mg/TID) for 14 days. Clinical and microbiological examinations were performed at baseline and at 6 months post-SRP. Six subgingival plaque samples per subject were analysed for the presence and levels of Archaea using quantitative polymerase chain reaction.

Results: Scaling and root planing alone or combined with MTZ or MTZ + AMX significantly reduced the prevalence of subjects colonized by Archaea at 6 months post-therapy, without significant differences among groups (P > .05). Both therapies led to a statistically significant decrease in the mean percentage of sites colonized by Archaea (P < .05). The MTZ and MTZ + AMX group had a significantly lower mean number of sites colonized by Archaea and lower levels of these micro-organisms at sites with probing depth ≥5 mm at 6 months compared with SRP group (P < .05).

Conclusion: Periodontal treatments including adjunctive MTZ or MTZ + AMX are more effective than mechanical treatment alone in reducing the levels and prevalence of sites colonized by Archaea in subjects with chronic periodontitis.

Keywords: archaea; chronic periodontitis; dental plaque; dental scaling; periodontitis; root planing.

Publication types

  • Randomized Controlled Trial

MeSH terms

  • Adult
  • Amoxicillin / administration & dosage*
  • Archaea / isolation & purification*
  • Biofilms*
  • Chronic Periodontitis / microbiology*
  • Chronic Periodontitis / therapy*
  • Combined Modality Therapy
  • Dental Plaque / microbiology*
  • Dental Scaling*
  • Drug Therapy, Combination
  • Female
  • Gingiva / microbiology*
  • Humans
  • Male
  • Metronidazole / administration & dosage*
  • Middle Aged
  • Root Planing*
  • Time Factors
  • Treatment Outcome

Substances

  • Metronidazole
  • Amoxicillin