Abstract
DAZ interacting zinc finger 3 (DZIP3), an RNA-binding RING-type ubiquitin ligase, has been reported to be involved in multiple physiological functions, including the regulation of chemokine- or estradiol-induced gene expression, self-renewal, and maintaining pluripotency in mouse embryonic stem cells. However, the precise cellular functions of DZIP3 remain elusive. In this study, we report the establishment of DZIP3-specific monoclonal antibodies (MAbs), using the rat medial iliac lymph node method. In immunoblotting analyses, our antibodies detected endogenous human and mouse DZIP3. In addition, immunoprecipitation analyses revealed the availability of these antibodies for human or mouse DZIP3. Thus, these MAbs will be available to elucidate cellular functions of DZIP3.
Keywords:
DZIP3; MAbs; the rat medial iliac lymph node method.
MeSH terms
-
Animals
-
Antibodies, Monoclonal / biosynthesis*
-
Antibodies, Monoclonal / chemistry
-
Antibodies, Monoclonal / isolation & purification
-
Cell Fusion
-
Cell Line
-
Cell Line, Tumor
-
Cloning, Molecular
-
Escherichia coli / genetics
-
Escherichia coli / metabolism
-
Female
-
Fibroblasts / cytology
-
Fibroblasts / immunology
-
Gene Expression
-
Genetic Vectors / chemistry
-
Genetic Vectors / metabolism
-
Humans
-
Hybridomas / immunology*
-
Immunization
-
Immunoblotting
-
Lymph Nodes / cytology
-
Lymph Nodes / immunology*
-
Mice
-
Multiple Myeloma / immunology
-
Multiple Myeloma / pathology
-
RNA-Binding Proteins / analysis*
-
RNA-Binding Proteins / genetics
-
RNA-Binding Proteins / immunology
-
Rats
-
Rats, Inbred WKY
-
Recombinant Fusion Proteins / administration & dosage
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / immunology
-
Ubiquitin-Protein Ligases / analysis*
-
Ubiquitin-Protein Ligases / genetics
-
Ubiquitin-Protein Ligases / immunology
Substances
-
Antibodies, Monoclonal
-
RNA-Binding Proteins
-
Recombinant Fusion Proteins
-
DZIP3 protein, human
-
Ubiquitin-Protein Ligases
-
Dzip3 protein, mouse