Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger

Morgann C Reilly; Saori Amaike Campen; Blake A Simmons; Scott E Baker; John M Gladden; Jon K Magnuson

doi:10.1007/978-1-4939-7877-9_10

Cloning and Expression of Heterologous Cellulases and Enzymes in Aspergillus niger

Methods Mol Biol. 2018:1796:123-133. doi: 10.1007/978-1-4939-7877-9_10.

Authors

Morgann C Reilly^{1

2}, Saori Amaike Campen^{1

3}, Blake A Simmons^{1

4}, Scott E Baker^{1

5}, John M Gladden^{1

6}, Jon K Magnuson^{7

8}

Affiliations

¹ Joint BioEnergy Institute, Emeryville, CA, USA.
² University of California, San Francisco, CA, USA.
³ J. Craig Venter Institute, San Diego, CA, USA.
⁴ Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
⁵ Pacific Northwest National Laboratory, Richland, WA, USA.
⁶ Sandia National Laboratory, Livermore, CA, USA.
⁷ Joint BioEnergy Institute, Emeryville, CA, USA. [email protected].
⁸ Pacific Northwest National Laboratory, Richland, WA, USA. [email protected].

PMID: 29856051
DOI: 10.1007/978-1-4939-7877-9_10

Abstract

Cellulases and other enzymes are needed for saccharification of plant biomass in the biorefinery industry. Expression, characterization, and eventual large-scale production of known and novel cellulases requires the ability to express and secrete heterologous enzymes in relevant protein production platforms like Aspergillus niger. A method for cloning and expression of genes for these desirable enzymes in A. niger is presented in this Chapter.

Keywords: Aspergillus niger; Cellulases; Enzymes; Heterologous protein expression; Protoplast transformation.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Aspergillus niger / enzymology*
Aspergillus niger / growth & development
Biomass
Cellulases / genetics*
Cellulases / metabolism
Cloning, Molecular / methods*
Protoplasts / metabolism
Transformation, Genetic

Substances

Cellulases