5' capped and 3' polyA-tailed sgRNAs enhance the efficiency of CRISPR-Cas9 system

Protein Cell. 2019 Mar;10(3):223-228. doi: 10.1007/s13238-018-0552-5.

Wei Mu^{1

2}, Yongping Zhang³, Xutong Xue⁴, Lei Liu^{1

2}, Xiaofei Wei⁵, Haoyi Wang⁶

¹ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
² University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Department of Hematology, Aerospace Center Hospital, Aerospace Clinical Medical College, Peking University, Beijing, 100049, China.
⁴ Hebei University, Baoding, 071002, China.
⁵ Beijing Cord Blood Bank, Beijing, 100044, China.
⁶ State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. [email protected].

No abstract available

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