"Cytoplasmic" islet cell antibodies. Evidence that the target antigen is a sialoglycoconjugate

Diabetes. 1985 Jun;34(6):617-9. doi: 10.2337/diab.34.6.617.

Abstract

We have biochemically treated (periodate, borohydride, neuraminidase, organic solvents) frozen sections of human pancreas and studied the reactivity of islet-cell-antibody-positive human sera and monoclonal antibodies. The autoantigen of pancreatic sections has the properties of sialic acid containing glycolipid.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies / analysis*
  • Antibodies, Monoclonal
  • Antigens / analysis*
  • Cytoplasm / immunology
  • Fluorescent Antibody Technique
  • Glycolipids / analysis*
  • Humans
  • Immunoenzyme Techniques
  • Islets of Langerhans / immunology*
  • Neuraminidase / metabolism
  • Periodic Acid
  • Pronase / metabolism
  • Sialic Acids / analysis*

Substances

  • Antibodies
  • Antibodies, Monoclonal
  • Antigens
  • Glycolipids
  • Sialic Acids
  • Periodic Acid
  • metaperiodate
  • Neuraminidase
  • Pronase