Extracellular Matrix Proteins Substantiate IL-28B T allele Effect on Histological Outcome of Chronic Hepatitis C

Ann Hepatol. 2018;17(4):569-576. doi: 10.5604/01.3001.0012.0918.

Abstract

Introduction and aim: The correlation between interleukin-28B (IL-28B) polymorphisms and chronic hepatitis C (CHC) progression is debatable. Here, we aimed to evaluate the relation between IL-28B C/T genotypes and the development of cirrhotic liver. Extracellular matrix (ECM) proteins, FibroScan and model for end-stage liver disease (MELD) were used to substantiate the severity of liver disease.

Material and methods: IL-28B rs12979860, liver stiffness and ECM proteins were assessed in 272 CHC patients.

Results: Cirrhosis percentage increased to 10%, 52% and 96% with the increasing number of T alleles (CC, CT and TT, respectively). Also, elevated ECM proteins levels were correlated with the increasing number of T alleles. Interestingly, among cirrhotic patients, liver stiffness, MELD and ECM proteins were significantly (P < 0.0001) higher in patients with TT more than CT genotype. FibroScan, hyaluronic acid, Laminin, Collagen IV and the N-terminal pro-peptide of collagen type III have high accuracy to differentiate liver status in CC from TT genotype. Area under receiver-operating characteristic curve (95% CI) were 1.0 (1.0-1.0), 0.97 (0.96- 1.0), 0.93 (0.85-1.0), 0.98 (0.97-1.0) and 0.93 (0.91-0.97), respectively.

Conclusion: This study suggests that IL-28B T allele affects the natural course of CHC type 4 and also suggests that carriage of the IL-28B C allele protects from unfavorable clinical outcomes in CHC as coexistence of C allele with T allele reduced cirrhosis severity.

Keywords: Chronic hepatitis; Cirrhosis; Fibrosis severity; Interleukin-28B; Polymorphisms.

MeSH terms

  • Disease Progression
  • Egypt
  • Elasticity Imaging Techniques
  • Extracellular Matrix Proteins / analysis*
  • Genetic Predisposition to Disease
  • Hepatitis C, Chronic / diagnosis
  • Hepatitis C, Chronic / genetics*
  • Hepatitis C, Chronic / metabolism*
  • Hepatitis C, Chronic / virology
  • Humans
  • Interferons
  • Interleukins / genetics*
  • Liver / chemistry*
  • Liver / diagnostic imaging
  • Liver / pathology
  • Liver / virology
  • Phenotype
  • Polymorphism, Single Nucleotide*
  • Prognosis
  • Protective Factors
  • Retrospective Studies
  • Risk Factors
  • Severity of Illness Index
  • Up-Regulation

Substances

  • Extracellular Matrix Proteins
  • interferon-lambda, human
  • Interleukins
  • Interferons