Abstract
Penicillin acylase is processed from a 90-kD precursor through the cleavage of a leader peptide and two further endopeptidase cleavages to yield an enzyme that contains a 22-kD (or 23-kD) and a 65-kD subunit. The endopeptidase cleavages require an intact carboxy terminus. This type of processing appears to be unique for a prokaryotic enzyme, having its most closely related analog in the synthesis and processing of preproinsulin and other eukaryotic hormones.
MeSH terms
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Amidohydrolases / genetics*
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Amino Acid Sequence
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Base Sequence
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Chromosome Mapping
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DNA Transposable Elements
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Genes, Bacterial
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Immunosorbent Techniques
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Molecular Weight
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Penicillin Amidase / genetics*
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Penicillin Amidase / immunology
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Penicillin Amidase / metabolism
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Promoter Regions, Genetic
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Protein Processing, Post-Translational*
Substances
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DNA Transposable Elements
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Amidohydrolases
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Penicillin Amidase
Associated data
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GENBANK/M11672
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GENBANK/M12373