Xpert Ultra Can Unambiguously Identify Specific Rifampin Resistance-Conferring Mutations

Kamela C S Ng; Armand van Deun; Conor J Meehan; Gabriela Torrea; Michèle Driesen; Siemon Gabriëls; Leen Rigouts; Emmanuel André; Bouke C de Jong

doi:10.1128/JCM.00686-18

Xpert Ultra Can Unambiguously Identify Specific Rifampin Resistance-Conferring Mutations

J Clin Microbiol. 2018 Aug 27;56(9):e00686-18. doi: 10.1128/JCM.00686-18. Print 2018 Sep.

Authors

Kamela C S Ng¹, Armand van Deun^{2

3}, Conor J Meehan², Gabriela Torrea², Michèle Driesen², Siemon Gabriëls², Leen Rigouts^{2

4}, Emmanuel André^#⁵, Bouke C de Jong^#²

Affiliations

¹ Unit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium [email protected].
² Unit of Mycobacteriology, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
³ International Union against Tuberculosis and Lung Disease, Paris, France.
⁴ Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.
⁵ Laboratory of Clinical Bacteriology and Mycology, KU Leuven, Leuven, Belgium.

^# Contributed equally.

No abstract available

Keywords: Ultra probes; Xpert MTB/RIF Ultra; disputed mutations; melt peak temperature (Tm); melting temperature shift (ΔTm); rifampin-resistant tuberculosis; rpoB mutations.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Antibiotics, Antitubercular / pharmacology
Bacterial Proteins / genetics*
DNA-Directed RNA Polymerases / genetics*
Drug Resistance, Bacterial / genetics*
Humans
Molecular Diagnostic Techniques / methods*
Molecular Probes
Mutation
Mycobacterium tuberculosis / genetics*
Mycobacterium tuberculosis / isolation & purification
Rifampin / pharmacology
Sensitivity and Specificity
Temperature
Tuberculosis / microbiology*

Substances

Antibiotics, Antitubercular
Bacterial Proteins
Molecular Probes
rpoB protein, Mycobacterium tuberculosis
DNA-Directed RNA Polymerases
Rifampin