Genome Sequences of 12 Mycobacteriophages Recovered from Archival Stocks in Japan

Jumpei Uchiyama; Keijiro Mizukami; Koji Yahara; Shin-Ichiro Kato; Hironobu Murakami; Tadahiro Nasukawa; Naoya Ohara; Midori Ogawa; Toshio Yamazaki; Shigenobu Matsuzaki; Masahiro Sakaguchi

doi:10.1128/genomeA.00472-18

Genome Sequences of 12 Mycobacteriophages Recovered from Archival Stocks in Japan

Genome Announc. 2018 Jun 21;6(25):e00472-18. doi: 10.1128/genomeA.00472-18.

Authors

Affiliations

¹ School of Veterinary Medicine, Azabu University, Kanagawa, Japan [email protected].
² School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
³ Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
⁴ Kochi University, Kochi, Japan.
⁵ Department of Oral Microbiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.
⁶ The Advanced Research Center for Oral and Craniofacial Sciences, Dental School, Okayama University, Okayama, Japan.
⁷ Department of Microbiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu City, Japan.
⁸ Division of Biosafety Control and Research, National Institute of Infectious Diseases, Tokyo, Japan.

Abstract

Using Mycobacterium smegmatis mc²155, 12 siphoviruses were recovered from long-term archival stocks stored in Japan. Their genome sequences were 46.0 to 61.3 kbp with 63 to 68% G+C contents, which allowed them to be categorized within cluster W and subclusters A1, A2, B3, A7, I1, and K4.