Abstract
Bovine brain, hypothalamus, pituitary, and retina contain potent anionic polypeptide mitogens for endothelial cells. Immunological assays using murine monoclonal antibodies against bovine endothelial cell growth factor (ECGF) and radioreceptor assays using [125I]ECGF were performed to determine the cross-reactivity of ECGF with bovine acidic pI brain-derived fibroblast growth factor (acidic FGF) and bovine eye-derived growth factor-II [EDGF-II). We observed that acidic FGF and EDGF-II are recognized by anti-ECGF monoclonal antibodies and compete with [125I] ECGF for receptor occupancy. Furthermore, the biological activity of ECGF, acidic FGF, and EDGF-II is potentiated by the glycosaminoglycan, heparin. These results argue that ECGF, acidic FGF, and EDGF-II belong to a common family of polypeptide growth factors.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antibody Specificity
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Binding, Competitive
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Brain Chemistry*
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Cattle
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Cell Division / drug effects
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Cells, Cultured
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Cross Reactions
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Drug Synergism
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Endothelial Growth Factors
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Endothelium / drug effects
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Epidermal Growth Factor / immunology
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Epidermal Growth Factor / metabolism
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Fibroblast Growth Factors / immunology
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Fibroblast Growth Factors / metabolism*
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Growth Substances / classification*
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Growth Substances / immunology
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Growth Substances / metabolism*
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Heparin / immunology
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Heparin / metabolism
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Heparin / pharmacology
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Humans
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Proteins
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Receptors, Cell Surface / metabolism
Substances
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Antibodies, Monoclonal
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Endothelial Growth Factors
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Growth Substances
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Proteins
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Receptors, Cell Surface
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eye-derived growth factor
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hypothalamus-derived growth factor
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Fibroblast Growth Factors
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Epidermal Growth Factor
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Heparin