Impact of patatin-like phospholipase domain containing 3 rs738409 G/G genotype on hepatic decompensation and mortality in patients with portal hypertension

Aliment Pharmacol Ther. 2018 Aug;48(4):451-459. doi: 10.1111/apt.14856. Epub 2018 Jun 29.

Abstract

Background: The rs738409 C>G p.I148M variant in the patatin-like phospholipase domain containing 3 (PNPLA3)-gene promotes triglyceride accumulation in hepatocytes and hepatic stellate cell activation and has previously been linked to hepatic steatosis/liver fibrosis.

Aim: To investigate its impact on hepatic decompensation and (liver-related) mortality in patients who had already developed portal hypertension. Moreover, we assessed its link with hepatic steatosis as evaluated by controlled attenuation parameter.

Methods: We performed a retrospective analysis in prospectively characterised patients with viral hepatitis/fatty liver disease-induced portal hypertension (hepatic venous pressure gradient [HVPG] ≥ 6 mm Hg) diagnosed at the Medical University of Vienna who underwent HVPG measurement (until 2013; n = 372; longitudinal study) or simultaneous HVPG and controlled attenuation parameter measurement (2014-2017; n = 153; cross-sectional study).

Results: While survival was similar between PNPLA3-C/C and -C/G patients, we observed substantially increased mortality in PNPLA3-G/G patients. PNPLA3-G/G had no impact on mortality in the subgroup of patients with viral hepatitis; however, we observed a strong independent association between PNPLA3-G/G and hepatic decompensation (adjusted subdistribution hazard ratio [aSHR]: 2.1, 95% confidence interval [95% CI]: 1.1-4; P = 0.024) as well as mortality (overall: aSHR: 2.2, 95% CI: 1.22-3.98; P = 0.009; liver-related: aSHR: 2.2, 95% CI: 1.08-4.46; P = 0.029) in patients with fatty liver disease. Interestingly, even in the subgroup of patients who had already progressed to clinically significant portal hypertension (HVPG ≥ 10 mm Hg), PNPLA3-G/G substantially increased mortality (aSHR: 2.33, 95% CI: 1.27-4.29; P = 0.006). PNPLA3-genotype had no influence on controlled attenuation parameter or the prevalence of values ≥248 dB/m.

Conclusion: PNPLA3-G/G-genotype seems to double the risks of hepatic decompensation and (liver-related) mortality in patients with portal hypertension due to fatty liver disease. Further studies are warranted to investigate potential underlying pathophysiological mechanisms unrelated to hepatic steatosis.

MeSH terms

  • Adult
  • Aged
  • Cross-Sectional Studies
  • Fatty Liver / complications
  • Fatty Liver / genetics
  • Fatty Liver / mortality
  • Female
  • Genetic Predisposition to Disease
  • Genotype
  • Hepatitis C, Chronic / complications
  • Hepatitis C, Chronic / genetics
  • Hepatitis C, Chronic / mortality
  • Hepatitis C, Chronic / pathology
  • Humans
  • Hypertension, Portal / complications
  • Hypertension, Portal / genetics*
  • Hypertension, Portal / mortality*
  • Lipase / genetics*
  • Liver Failure / complications
  • Liver Failure / genetics*
  • Liver Failure / mortality*
  • Longitudinal Studies
  • Male
  • Membrane Proteins / genetics*
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Retrospective Studies
  • Survival Rate

Substances

  • Membrane Proteins
  • Lipase
  • adiponutrin, human