High-throughput sequencing and bioinformatic techniques have enhanced classical genetic analysis and are essential methods for geneticists. Tsukamoto and colleagues use numerous genomic and bioinformatics methods to explore the role of ribonucleoprotein complexes in regulating oocyte meiotic maturation, which is the transition between diakinesis and metaphase of meiosis I. This primer provides guidance for both educators and students as they read "LIN-41 and OMA Ribonucleoprotein Complexes Mediate a Translational Repression-to-Activation Switch Controlling Oocyte Meiotic Maturation and the Oocyte-to-Embryo Transition in Caenorhabditis elegans" The primer provides background information on the utility of the C. elegans germ line as a model for meiotic regulation, and further describes methods of bioinformatic analysis used to study translational and post-translational gene regulation. Additionally, the primer provides discussion questions and an active learning exercise designed to enhance student learning of critical genetic concepts.
Keywords: C. elegans germ line; education; high-throughput bioinformatic analysis; oocyte meiotic maturation; ribonucleoprotein complex.
Copyright © 2018 by the Genetics Society of America.