Abstract
Yeast libraries revolutionized the systematic study of cell biology. To extensively increase the number of such libraries, we used our previously devised SWAp-Tag (SWAT) approach to construct a genome-wide library of ~5,500 strains carrying the SWAT NOP1promoter-GFP module at the N terminus of proteins. In addition, we created six diverse libraries that restored the native regulation, created an overexpression library with a Cherry tag, or enabled protein complementation assays from two fragments of an enzyme or fluorophore. We developed methods utilizing these SWAT collections to systematically characterize the yeast proteome for protein abundance, localization, topology, and interactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Genetic Complementation Test
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Genome, Fungal*
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Genomic Library*
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Mitochondrial Proteins / genetics
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Mitochondrial Proteins / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Promoter Regions, Genetic
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Protein Interaction Mapping
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Proteome / genetics*
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Proteome / metabolism
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Ribonucleoproteins, Small Nucleolar / genetics
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Ribonucleoproteins, Small Nucleolar / metabolism
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Sequence Tagged Sites
Substances
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Mitochondrial Proteins
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NOP1 protein, S cerevisiae
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Nuclear Proteins
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Proteome
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Recombinant Fusion Proteins
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Ribonucleoproteins, Small Nucleolar
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins