This paper describes the synthesis and catalytic activities of stratiform protein microtube reactors containing a glucose oxidase (GOD) enzyme layer. The microtubes were fabricated by layer-by-layer assembly using a microporous polycarbonate membrane with human serum albumin (HSA), poly(l-arginine) (PLA), and GOD. The GOD component was introduced into the tube wall as the innermost layer, the intermediate layer, or all internal protein layers. SEM observations revealed the formation of uniform hollow cylinders with ca. 1.17 μm outer diameter and ca. 135 nm wall thickness. In aqueous medium, each microtube catalyzed β-d-glucose oxidation with high efficiency. We first ascertained the enzyme parameters (Km and kcat ) of these microtube reactors. Different catalytic activities that have dependent on the GOD layer position in the cylindrical wall have been elucidated.
Keywords: enzymes; layer-by-layer assembly; microreactors; multilayered structure; proteins.
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